Updated: 12 September 2005
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1. Aquasmart water filter cartridges (Cryptosporidium removed)
2. Aureon Bioproducts (Cryptosporidium diagnostic kits)
3. Becton, Dickinson and Company\ (Cryptosporidium direct testing and serology)
4. Biosite triage parasite panel (Cryptosporidium screening)
5. Cellabs (Cryptosporidium Cel diagnostic kits)
6. Conway corporation (Water testing)
7. Cypress Diagnostics (Veterinary diagnostic kits)
8. Espitech water filters (Cryptosporidium removed)
9. Excelsior Sentinel Inc. (Cryptosporidium oocyst-loaded sentinel chambers)
10. Great Smokies Diagnostics (Diagnostic company)
11. Hydro-photon Inc. (Ultraviolet SteriPen)
12. Innowave 240 (Cryptosporidium removed)
13. Immtech Inc. (Distributor for Aureon bioproducts)
14. ImmuCell (Water testing)
15. Northern Mountain supply SweetWater filters (Cryptosporidium removed)
16. O3 Water Systems, LLC. (Cryptosporidium removed)
17. Pure Earth water filters (Cryptosporidium removed)
18. RDI Research Diagnostics Inc. (Cryptosporidium antibodies)
19. SafePath Labs (LMD AgroVet Cryptosporidium diagnostic kits)
20. Strategic Diagnostics (HydroFluor Combo for Cryptosporidium)
21. TCS Biosciences (Diagnostic kits)
22. Turner parasitology (Parasite diagnosis)
23. Waterborne Inc. (AquaGlo Cryptosporidium detection kit)
24. WaterHealth International (UV disinfection)
    

ATCC 87439 Cryptosporidium parvum gDNA library, KSU-1 isolate originally from calf near Bendena, Kansas. Genotype 2 (C). Host animal: calf; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 22 Aug. 1996 by NV Khramtsov & SJ Upton.

ATCC 87664 Cryptosporidium serpentis gDNA library, KSU-2 isolate from captive snake in Kansas. Host animal: corn snake (Elaphe guttata) from a private collection in Kansas; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 17 Dec. 1997 by NV Khramtsov & SJ Upton.

ATCC 87665 Cryptosporidium parvum gDNA library, GCH1 isolate from Tufts University. Predominately genotype 2 (C); the literature reports that some genotype 1 (H) may be a minor component. Viable oocysts sold through the NIH AIDS reagent program as catalog number 2259. Host animal: calf (originally human); Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 17 Dec. 1997 by NV Khramtsov & SJ Upton.

ATCC 87666 Cryptosporidium muris gDNA library, 108735 isolate, originally from National Park Zoo, Virginia. Host animal: 15th passage in mouse (originally isolated from a rock hyrax, however); Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 17 Dec. 1997 by NV Khramtsov & SJ Upton.

ATCC 87667 Cryptosporidium parvum gDNA library, Iowa (A) isolate originally from Iowa and maintained at the Univerisity of Arizona. Genotype 2 (C). Viable oocysts sold through the NIH AIDS reagent program as catalog number 1372. The isolate remained separate from the Iowa (I) isolate (ATCC 87668) for at least a dozen years prior to DNA extraction. Host animal: calf; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 17 Dec. 1997 by NV Khramtsov & SJ Upton.

ATCC 87668 Cryptosporidium parvum gDNA library, Iowa (I) isolate originally from Iowa and maintained at Pleasant Hill Farms in Idaho. Genotype 2 (C). The isolate remained separate from the Iowa (A) isolate (ATCC 87667) for over a dozen years prior to DNA extraction. Host animal: calf; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 17 Dec. 1997 by NV Khramtsov & SJ Upton.

ATCC 87712 Cryptosporidium parvum gDNA library, UCP (=Ungar Cryptosporidium parvum) isolate thought to be derived from the Iowa isolate in 1990 and currently maintained at ImmunoCell Inc. Genotype 2 (C). It remained separate from the Iowa (A) (ATCC 87667) and Iowa (I) (ATCC 87668) isolates for at least 8 years (perhaps longer) prior to DNA extraction. Host animal: calf; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 07 July 1998 by NV Khramtsov & SJ Upton.

ATCC 87713 Cryptosporidium parvum gDNA library, Louisiana-1 isolate originally obtained from a calf in 1997 and currently maintained by Waterborne Technologies Inc., New Orleans. Genotype 2 (C). Host animal: calf; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 07 July 1998 by NV Khramtsov & SJ Upton.

ATCC 87714 Cryptosporidium parvum gDNA library, Cornell-1 isolate, collected in July 1997 by Dr. Rosemary Soave from an AIDS patient at the Cornell University Medical Center. Genotype 2 (C). Host animal: Homo sapiens; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 07 July 1998 by NV Khramtsov & SJ Upton.

ATCC 87715 Cryptosporidium andersoni gDNA library, VS1742 isolate; large abomasal species sometimes termed "C. muris-like" collected from a 3.5 yr old heifer near Caldwell, Idaho on 10 Dec. 1996 by Dr. Bruce Anderson. Low level eimerian contamination noted. Host animal: Bos taurus; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 07 July 1998 by NV Khramtsov & SJ Upton.

ATCC 87716 Cryptosporidium andersoni gDNA library, 95-400 isolate; large abomasal species sometimes termed "C. muris-like" collected from a 2 yr old beef steer in Ada county, Idaho in 1997 by Dr. Bruce Anderson. Host animal: Bos taurus; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Date submitted: 07 July 1998 by NV Khramtsov & SJ Upton.

ATCC 87760 Cryptosporidium parvum gDNA library, Iowa 395 (also termed Iowa A) isolate originally from Iowa and maintained at the University of Arizona. Sold through the NIH AIDS reagent program as catalog number 1372 and the same isolate as ATCC 87667. Genotype 2 (C). Host animal: calf; Vector: phagemid lambda Zap express; Vector ends: Sau3A I partial; Suggested host: Escherichia coli XL1-blue MRF; Submitted: September 1999 by C.C. Dykstra.

ATCC 87761 Cryptosporidium parvum gDNA library, GCH1 (also termed GCH1 995) isolate from Tufts University. Sold through the NIH AIDS reagent program as catalog number 2259 and the same isolate as ATCC 87665. Predominately genotype 2 (C); the literature reports that some genotype 1 (H) may be a minor component. Host animal: calf (originally human); Vector: phagemid lambda Zap express; Vector ends: Sau3A I partial; Suggested host: Escherichia coli XL1-blue MRF; Submitted: September 1999 by C.C. Dykstra

ATCC 87762 Cryptosporidium parvum gDNA library, AUCp2 isolate from Auburn University. Genotype 2. Host animal: calf (isolated in August, 1996 at Auburn University); Vector: phagemid lambda Zap express; Vector ends: Sau3A I partial; Suggested host: Escherichia coli XL1-blue MRF; Submitted: September 1999 by C.C. Dykstra

ATCC 87763 Cryptosporidium parvum gDNA library, Galicia isolate, originally from a Friesian calf in Galicia, NW Spain by Dr. Elvira Ares-Mazas in July, 1998. Unsporulated oocysts of an eimerian was also noted microscopically. Genotype 2 (C). Host animal: calf; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Submitted: November 1999 by NV Khramtsov and SJ Upton.

ATCC 87764 Cryptosporidium andersoni gDNA library, VMTRC49 isolate; large abomasal species sometimes termed "C. muris-like" collected originally in 1998 from an adult Holstein dairy cow near Tulare, California by Dr. E.R. Atwill. Host animal: Bos taurus; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Submitted: November 1999 by NV Khramtsov and SJ Upton.

ATCC 87765 Cryptosporidium parvum gDNA library, Colombia isolate, originally from calf near Medellin, Colombia by Dr. Elvira Ares-Mazas in October, 1998. Genotype 2 (C). Host animal: calf; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Submitted: November 1999 by NV Khramtsov and SJ Upton.

ATCC 87766 Cryptosporidium parvum gDNA library, Texas isolate,originally from an AIDS patient in Texas by Drs C.L. Chappell & P.C. Okhuysen in 1998. Genotype 1 (H). Host animal: Homo sapiens; Vector: pBluescript II SK+; Vector ends: EcoR1; Host range: Escherichia coli; Submitted: November 1999 by NV Khramtsov and SJ Upton.

ATCC 87767 Cryptosporidium parvum gDNA library, Texas isolate, originally from an AIDS patient in Texas by Drs C.L. Chappell & P.C. Okhuysen in 1998. Genotype 1 (H). Host animal: Homo sapiens; Vector: pBluescript II SK+; Vector ends: HindIII; Host range: Escherichia coli; Submitted: November 1999 by NV Khramtsov and SJ Upton.

ATCC 87768 Cryptosporidium parvum gDNA library, KSU-1 isolate originally from calf near Bendena, Kansas in 1987. Same isolate as ATCC 87439. Genotype 2 (C). Host animal: calf; Vector: pBluescript II SK+; Vector ends: HindIII; Host range: Escherichia coli; Submitted: November 1999 by NV Khramtsov and SJ Upton.

NIH AIDS Reagent 1372 Cryptosporidium parvum viable oocysts, Iowa isolate maintained at the University of Arizona. Host animal: calf

NIH AIDS Reagent 1491 Hyperimmune bovine colostrum, irradiated.

NIH AIDS Reagent 1558 Cryptosporidium parvum, no isolate designated; Host animal: calf; Other: Two primers supplied in water for amplification of parasite DNA as cited in Laxer et al., 1991, Am. J. Trop. Med. Hyg. 45: 688-694

NIH AIDS Reagent 1559 Cryptosporidium parvum, no isolate designated; Host animal: calf; Vector: DH5-alpha bacteria; Vector ends: HindIII; Other: Bacteria contain a 452 bp PCR amplification target as cited in Laxer et al., 1991, Am. J. Trop. Med. Hyg. 45: 688-694

NIH AIDS Reagent 1644 Cryptosporidium parvum gDNA library, GCH1 isolate from Tufts University. Host animal: calf (originally from human); Vector: lambda ZAPII; Vector ends: EcoR1, partial digest; Host range: Escherichia coli XL-1 Blue or SURE

NIH AIDS Reagent 1897 Cryptosporidium parvum cDNA library, GCH1 isolate from Tufts University. Host animal: calf (originally from human); Vector: lambda ZAPII; Vector ends: EcoR1-XhoI; Host range: Escherichia coli XL-1 Blue or SURE

NIH AIDS Reagent 2259 Cryptosporidium parvum viable oocysts, GCH1 isolate maintained at Tufts University. Host animal: calf

UK HGMP Cryptosporidium parvum PAC library Moredun cervine isolate. Host animal: lambs (originally deer); Vector: pCYPACII; Vector ends: MboI partial digest; Host range: Escherichia coli DH10-B; Other: Average insert size 38 kb; library consists of 1602 clones providing about 6 x genome coverage.

1. American Type Culture Collection
2. NIH AIDS Reagent Program
3. UK Human genome mapping project resource center

1. 49 CFR transportation regulations
   
2. Canadian transport of dangerous goods database
   
3. CDC Office of Health & Safety regulations
   
4. HAZMAT
   
5. Shipping hazardous materials
   

Steve J. Upton, PhD
Division of Biology, Ackert Hall
Kansas State University
Manhattan, KS 66506