Michal Zolkiewski, Ph.D., Professor
Chair Graduate Biochemistry Group
Undergraduate Advisor
Complete Publications List
1980s
Antosiewicz, J., Hoiland, H., Zolkiewski, M. and Shugar, D.: "Quasichemical Interpretation of the Ultrasonic Velocity in Ternary Aqueous Systems", J. Solution Chem., 16, 285-294, 1987.
Zolkiewski, M.: "Kirkwood-Buff Integrals and Density Fluctuations in Aqueous Solution of Caffeine", J. Solution Chem., 16, 1025-1034, 1987.
Zolkiewski, M., Krestov, G.A., V'yugin, A.I. and Zielenkiewicz, W.: "Enthalpies of Solution of 1,3-dimethyluracil and 1,3-diethylthymine in Water - N,N-dimethylformamide Mixtures", Bull. Pol. Acad. Sci. Chem., 37, 433-438, 1989.
1990s
Zolkiewski, M. and Zielenkiewicz, W.: "Enthalpies of Solvation of Alkylated Uracils in Water, Nonaqueous and Mixed Solvents", J. Solution Chem., 20, 517-530, 1991.
Kulinski, T., Bratek-Wiewiorowska, M.D., Wiewiorowski, M., Zielenkiewicz, A., Zolkiewski, M. and Zielenkiewicz, W.: "Comparative Calorimetric Studies on the Dynamic Conformation of Plant 5S rRNA: Structural Interpretation of the Thermal Unfolding Patterns for Lupin Seeds and Wheat Germ", Nucl. Acids Res., 19, 2449-2455, 1991.
Zielenkiewicz, A., Zolkiewski, M., Zielenkiewicz, W. and Wiewiorowski, M.: "The Conformational Changes of 5S rRNA of Plant Origin in Presence of Anions PO4 -3, NO3 -, ClO4 -, Cl-, of tetra-Protonated Spermine and Magnesium Salts by Adiabatic Scanning Differential Calorimetry", Thermochim. Acta, 182, 165-174, 1991.
Wiewiorowski, M., Zielenkiewicz, A., Zielenkiewicz, W. and Zolkiewski, M.: "The Conformational Changes of 5S rRNA of Plant Origin in Presence of Sperminium and Spermidinium Cations by Adiabatic Scanning Differential Calorimetry", Thermochim. Acta, 182, 153-164, 1991.
Wiewiorowski, M., Zielenkiewicz, A., Zielenkiewicz, W. and Zolkiewski, M.: "The Conformational Changes of 5S rRNA of Plant Origin in Presence of Magnesium Cations by Adiabatic Scanning Differential Calorimetry", Thermochim. Acta, 182, 143-152, 1991.
Ginsburg, A. and Zolkiewski, M.: "Differential Scanning Calorimetry Study of Reversible, Partial Unfolding Transitions in Dodecameric Glutamine Synthetase from Escherichia coli", Biochemistry, 30, 9421-9429, 1991.
Zolkiewski, M. and Ginsburg, A.: "Thermodynamic Effects of Active-Site Ligands on the Reversible, Partial Unfolding of Dodecameric Glutamine Synthetase from Escherichia coli: Calorimetric Studies", Biochemistry, 31, 11991-12000, 1992.
Ginsburg, A. and Zolkiewski, M.: "Temperature and Guanidine Induced Unfolding of Dodecameric Glutamine Synthetase from E. coli", Pure Appl. Chem., 66, 469-472, 1994.
Redowicz, M.J., Martin, B., Zolkiewski, M., Ginsburg, A. and Korn, E.D.: "Effects of Phosphorylation and Nucleotides on the Conformation of Myosin II from Acanthamoeba castellanii", J. Biol. Chem., 269, 13558-13563, 1994.
Zolkiewski, M., Redowicz, M.J., Korn, E.D. and Ginsburg, A.: "Thermally Induced Unfolding of Acanthamoeba Myosin II and Skeletal Muscle Myosin: Nucleotide Effects", Arch. Biochem. Biophys., 318, 207-214, 1995.
Zolkiewski, M., Nosworthy, N.J. and Ginsburg, A.: "Urea Induced Dissociation and Unfolding of Dodecameric Glutamine Synthetase from Escherichia coli: Calorimetric and Spectral Studies", Protein Sci., 4, 1544-1552, 1995.
Zolkiewski, M., Redowicz, M.J., Korn, E.D. and Ginsburg, A.: "Thermal Unfolding of Acanthamoeba Myosin II and Skeletal Muscle Myosin", Biophys. Chem., 59, 365-371, 1996.
Zolkiewski, M., Redowicz, M.J., Korn, E.D., Hammer, J.A. III and Ginsburg, A.: “A Two-State Unfolding of a Long Dimeric Coiled-Coil: the Acanthamoeba Myosin II Rod”, Biochemistry, 36, 7876-7883, 1997.
Redowicz, M.J., Hammer, J.A., Bowers, B., Zolkiewski, M., Ginsburg, A., Korn, E.D. and Rau, D.C.: “Flexibility of Acanthamoeba Myosin Rod Minifilaments”, Biochemistry, 38, 7243-7252, 1999.
Zolkiewski, M., Kessel, M., Ginsburg, A. and Maurizi, M.R.: “Nucleotide-dependent oligomerization of ClpB from Escherichia coli”, Protein Sci., 8, 1899-1903, 1999.
Zolkiewski, M.: “ClpB cooperates with DnaK, DnaJ, and GrpE in suppressing protein aggregation: a novel multi-chaperone system from Escherichia coli”, J. Biol. Chem., 274, 28083-28086, 1999.
2000s
Gong, X., Peng, T., Yakhnin, A., Zolkiewski, M., Quinn, J., Yeamen, S.J. and Roche, T.E.: “Specificity Determinants for the Pyruvate Dehydrogenase Component Reaction Mapped with Mutated and Prostethic Group Modified Lipoyl Domains”, J. Biol. Chem., 275, 13645-13653, 2000.
Zheng, L., Krishnamoorthi, R., Zolkiewski, M. and Wang, X.: “Distinct Ca2+-Binding Properties of Novel C2 Domains of Plant Phospholipase D alpha and beta”, J. Biol. Chem., 275, 19700-19706, 2000.
Barnett, M.E., Zolkiewska, A. and Zolkiewski, M.: “Structure and Activity of ClpB from Escherichia coli: Role of the Amino- and Carboxy-terminal Domains”, J. Biol. Chem., 275, 37565-37571, 2000.
Zavodszky, M., Chen, C.W., Huang, J.K., Zolkiewski, M., Wen, L. and Krishnamoorthi, R.: “Disulfide Bond Effects on Protein Stability: Designed Variants of Cucurbita maxima Trypsin Inhibitor-V”, Protein Sci., 10, 149-160, 2001.
Urbauer, J.L., Adelman, K., Bieber Urbauer, R.J., Simeonov, M.F., Gilmore, J.M., Zolkiewski, M. and Brody, E.N.: "Conserved Regions 4.1 and 4.2 of σ70 Constitute the Recognition Sites for the Anti-σ Factor AsiA, and AsiA Is a Dimer Free in Solution", J. Biol. Chem., 276, 41128-41132, 2001.
Tek, V. and Zolkiewski, M.: "Stability and Interactions of the Amino-terminal Domain of ClpB from Escherichia coli", Protein Sci., 11, 1192-1198, 2002.
Liu, Z., Tek, V., Akoev, V. and Zolkiewski, M.: "Conserved Amino-acid Residues within the Amino-terminal Domain of ClpB are Essential for the Chaperone Activity", J. Mol. Biol., 321, 111-120, 2002.
Barnett, M.E. and Zolkiewski, M.: "Site-directed Mutagenesis of Conserved Charged Amino-acid Residues in ClpB from Escherichia coli", Biochemistry, 41, 11277-11283, 2002.
Kedzierska, S., Akoev, V., Barnett, M.E. and Zolkiewski, M.: “Structure and Function of the Middle Domain of ClpB from Escherichia coli”, Biochemistry, 42, 14242-14248, 2003.
Liu, Z., Zolkiewska, A. and Zolkiewski, M.: "Characterization of human torsinA and its dystonia-associated mutant form", Biochem., 374(Pt 1), 117-122, 2003.
Akoev, V., Gogol, E.P., Barnett, M.E. and Zolkiewski, M.: "Nucleotide-Induced Switch in Oligomerization of the AAA+ ATPase ClpB", Protein Sci., 13, 567-574, 2004.
Chow, I.T., Barnett, M.E., Zolkiewski, M. and Baneyx. F.: "The N-terminal domain of Escherichia coli ClpB enhances chaperone function", FEBS Lett., 579, 4242-8, 2005.
Barnett, M.E., Nagy, M., Kedzierska, S. and Zolkiewski, M.: "The Amino-Terminal Domain of ClpB Supports Binding to Strongly Aggregated Proteins", J. Biol. Chem., 280, 34940-34945, 2005.
Kedzierska, S., Chesnokova, L.S., Witt, St.N., and Zolkiewski, M.: "Interactions within the ClpB/DnaK bi-chaperone system from Escherichia coli", Archives of Biochemistry and Biophysics, 444, 61-65, 2005.
Modrak-Wojcik, A., Stepniak, K., Akoev, V., Zolkiewski, M., and Bzowska, A.: "Molecular architecture of E. coli purine nucleoside phosphorylase studied by analytical ultracentrifugation and CD spectroscopy", Protein Science, 15, 1794-1800, 2006.
Zolkiewski, M.: “A camel passes through the eye of a needle: protein unfolding activity of Clp ATPases”, Mol. Microbiol., 61, 1094-1100, 2006.
Nagy, M., Akoev, V. and Zolkiewski, M.: “Domain Stability in the AAA+ ATPase ClpB from Escherichia coli”, Arch. Biochem. Biophys., 453, 63-69, 2006.
Doyle, S.M., Shorter, J., Zolkiewski, M., Hoskins, J.R., Lindquist, S. and Wickner, S.: “Asymmetric deceleration of ClpB and Hsp104 ATPase activity unleashes protein-remodeling activity”, Nature Struct. Mol. Biol., 14, 114-122, 2007.
Zolkiewski, M.: "ClpB: a chaperone for protein disaggregation", in Houry, W. (ed.), Molecular Chaperones: Principles and Diseases, The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd, London, 2007.
Nagy, M., Wu, H.-C., Liu, Z., Kedzierska-Mieszkowska, S. and Zolkiewski, M.: “Walker-A threonine couples nucleotide occupancy with the chaperone activity of the AAA+ ATPase ClpB”, Protein Sci., 18, 287-293, 2009.
2010s
Nagy, M., Guenther, I., Akoyev, V., Barnett, M.E., Zavodszky, M.I., Kedzierska-Mieszkowska, S. and Zolkiewski, M.: “Synergistic cooperation between two ClpB isoforms in aggregate reactivation”, J. Mol. Biol., 396, 697-707, 2010.
Gilmore, J.M., Bieber Urbauer, R.J., Minakhin, L., Akoyev, V., Zolkiewski, M., Severinov, K. and Urbauer J.L.: "Determinants of affinity and activity of the anti-sigma factor AsiA", Biochemistry, 49(29), 6143-54, 2010.
Zolkiewski, M. and Wu, H.-C.: “Emerging Area: TorsinA, a Novel ATP-Dependent Factor Linked to Dystonia” in Witt, S. N. (ed.), Protein Chaperones and Protection from Neurodegenerative Diseases, Wiley, 2011.
Zolkiewski M., Zhang, T. and Nagy, M.: "Aggregate reactivation mediated by the Hsp100 chaperones" Arch Biochem Biophys., 520, 1-6, 2012.
Zhang, T., Ploetz, E.A., Nagy, M., Doyle, S.M., Wickner, S., Smith, P.E. and Zolkiewski, M.: "Flexible connection of the N-terminal domain in ClpB modulates substrate binding and the aggregate reactivation efficiency", Proteins, 80, 2758-68, 2012.
Guenther, I., Zolkiewski, M. and Kędzierska-Mieszkowska, S.: "Cooperation between two ClpB isoforms enhances the recovery of the recombinant β-galactosidase from inclusion bodies", Biochem Biophys REs Commun., 426, 596-600, 2012.
An, C., Hiromasa, Y., Zhang, X., Lovell, S., Zolkiewski, M., Tomich, J.M. and Michel. K.: "Biochemical characterization of Anopheles gambiae SRPN6, a malaria parasite invasion marker in mosquitoes", PLoS One, 7(11):e48689, 201
Park, S., Li, X., Kim, H.M., Singh, C.R., Tian, G., Hoyt, M.A., Lovell, S., Battaile, K.P., Zolkiewski, M., Coffino, P., Roelofs, J., Cheng, Y. and Finley, D.: "Reconfiguration of the proteasome during chaperone-mediated assembly", Nature, 497, 512-6, 2013.
Zhang, T., Kedzierska-Mieszkowska, S., Liu, H., Cheng, C., Ganta, R.R. and Zolkiewski, M.: "Aggregate-reactivation activity of the molecular chaperone ClpB from Ehrlichia chaffeensis", PLoS One, 8(5):e62454, 2013.
Ngansop, F., Li, H., Zolkiewska, A. and Zolkiewski, M.: "Biochemical characterization of the apicoplast-targeted AAA+ ATPase ClpB from Plasmodium falciparum", Biochem Biophys Res Commun., 469, 191-5, 2013.
Zacchi, L.F., Wu, H.C., Bell, S.L., Millen, L., Paton, A.W., Paton, J.C., Thomas, P.J., Zolkiewski, M. and Brodsky, J.L.: "The BiP Molecular Chaperone Plays Multiple Roles during the Biogenesis of TorsinA, an AAA+ ATPase Associated with the Neurological Disease Early-onset Torsion Dystonia", J Biol Chem., 289, 12727-47, 2014.
Wielgus-Kutrowska, B., Modrak-Wójcik, A., Dyzma, A., Breer, K., Zolkiewski, M. and Bzowska, A.: "Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme", Arch Biochem Biophys., 549, 40-8, 2014.
Zacchi, L. F., Wu, H.-C., Bell, S. L., Millen, L., Paton, A. W., Paton, J. C., Thomas, P. J., Zolkiewski, M., and Brodsky, J. L.: (2014) “The BiP molecular chaperone plays multiple roles during the biogenesis of TorsinA, a AAA+ ATPase associated with the neurological disease Early-Onset Torsion Dystonia”, J. Biol. Chem., 289:12727-12747.
Zblewska, K., Krajewska, J., Zolkiewski, M., and kedzierska-Mieszkowska, s.; (2014) "Role of the disaggregase ClpB in processing of proteins aggregated as inclusion bodies", Arch. Biochem. Biophys. 555/556C:23/27
Woehl, JL., Stapels, DA., Garcia, BL., Ramyar, KX., Keightley, A., Ruyken, M., Syriga, M., Sfyroera, G., Weber, AB., Zolkiewski, M., Ricklin, D., Lambris, JD., Rooijakkers, SH., and Geisbrecht, BV. (2014) “The Extracellular Adherence Protein from Staphylococcus aureus Inhibits the Classical and Lectin Pathways of Complement by Blocking Formation of the C3 Proconvertase.” J Immunol Nov 7. Pii:1401600. [Epub ahead of print] This paper was selected to be highlighted in the “In This Issue” section of the journal.
Zhang, X., Meekins, DA., An, C., Zolkiewski, M., Battaile, KP., Kanost, MR., Lovell, S., and Michel, K. (2014) "Structural and Inhibitory Effects of Hinge Loop Mutagenesis in Serpin-2 the Malaria Vector Anopheles gambiae." J Biol Chem. Dec 14. pii:jbc.M114.625665
Li, H., Wu, H-C., Liu, Z., Zacchi, LF., Brodsky, JL., and Zolkiewski, M. (2014) "Intracellular Complexes of the Early-Onset Torsion Dystonia-Associated AAA+ ATPase TorsinA" SpringerPlus, 3:743
Zolkiewski, M., Chesnokova L. S., and Witt, S. N.: “Reactivation of Aggregated Proteins by the ClpB/DnaK Bi-chaperone System”, Curr. Protoc. Protein Sci. 83:28.10.1-28.10.18. doi: 10.1002/0471140864.ps2810s83, 2016.
Wise R, Duhachek-Muggy S, Qi Y, Zolkiewski M, Zolkiewska A. Protein disulfide isomerases in the endoplasmic reticulum promote anchorage-independent growth of breast cancer cells. Breast Cancer Res Treat. 2016 Jun;157(2):241-252.