INTRODUCTION

1. Introduction: Genetics

PART A : YEAST GENETICS: BACKGROUND

1. Baker's Yeast and its Lifecycle
2. Genetics of Baker's Yeast
3. The Response of Yeast to Radiation

PART B : BASIC GENETICS EXPERIMENTS

1. Simple Cross
2. Two genes/One Trait
3. A Dihybrid Cross
4. A Genetic Test for Allelism
5. Environmental Effects on Colony Color
6. The Yeast Mating Pheromone
7. Yeast Transformation
8. Plasmid Loss

PART C : UV EXPERIMENTS

1. Observing the Effects of Solar Ultraviolet Radiation on Cells
2. Serial Dilution and Viable Cell Counts
3. Using Yeast to Measure the Intensity of Solar Ultraviolet Radiation
4. Ultraviolet Lethality and Mutations in Yeast
5. Repair of Ultraviolet Damage: Photoreactivation
6. Ultraviolet Induction of Mitotic Segregation

PART D : MUTATION EXPERIMENTS

1. Spontaneous Mutation : Isolating "White" Mutants
2. Analysis of "White" Mutants of Red Strains
3. Testing Early AMP Pathway Mutants for Allelism
4. Separating the White Adenine Mutants from the Red Ones
5. Hunting for Red Mutants

PART E : ANALYSIS OF MEIOTIC PRODUCTS

1. Genetic Analysis of Ascospores by a Complementation Test
2. Genetic Analysis of Free Ascospores
3. Genetic Analysis of Intact Asci

PART F : A CLOSER LOOK AT .....ESSAYS

1. A Closer Look at...Adenine-requiring Mutants
2. A Closer Look at...Genetics Transformation of Yeast
3. A Closer Look at...Ultraviolet Radiation in our Environment
4. A Closer Look at...Stratospheric Ozone
5. A Closer Look at...Biological Consequences of Ultraviolet Exposure
6. A Closer Look at...Repair of DNA
7. A Closer Look at...Designing Quantitative Experiments
8. A Closer Look at...The Dilution Series and Statistics
9. A Closer Look at...Analyzing Survival Data
10. A Closer Look at...Describing Amounts of Work or Energy
11. A Closer Look at...Light and Energy
12. A Closer Look at...Modeling the Effects of Ultraviolet Radiation

PART G : YEAST LABORATORY METHODS AND MATERIALS

1. Laboratory Methods
2. Instructions for Building a UV Radiation Chamber

PART G : GLOSSARY AND REFERENCES

1. Glossary
2. References

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Kansas State University
November 25, 2002