Protocols and Methodology

Listed below are protocols related to lipid profiling. These methods have been optimized for lipidomics analyses.

• Extraction Method for Arabidopsis and Other Leaves for Lipid Profiling (3 methods - NEW: Videos for Methods 1 and 2)
• Extraction Method for Arabidopsis Seeds for Lipid Profiling
• Extraction Method for Animal Tissue for Lipid Profiling
• Extraction Method for Yeast for Lipid Profiling
• Q-Test

Acquisition methods for the KLRC's mass spectrometer set-ups are available:

• Acquisition methods

Instrument acquisition at Kansas Lipidomics Research Center has been supported by National Science Foundation (EPS 0236913, DBI 0521587, DBI1228622, and DBI1726527), Kansas Technology Enterprise Corporation, K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institute of Health (P20GM103418), and Kansas State University. Research in the Welti group (including method development) has been supported by National Science Foundation. Any opinions, findings, and conclusions or recommendations expressed in this web material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation or other granting agencies.