PEPTIDE/PROTEIN SYNTHESIS:
Two scales of synthesis are availiable (0.1 mmole and 0.5 mmole).
Generally fmoc chemistry is used, but
t-boc chemistry is availiable upon request. Fmoc chemistry is limited
to peptides up
to 35 residues in length.
Set-up
fee for fmoc or t-boc
synthesis
0.1 mmole t-boc
chemistry/residue
0.5 mmole t-boc
chemistry/residue
0.1 mmole fmoc
chemistry/residue
0.5 mmole fmoc
chemistry/residue
peptide cleavage and analytic HPLC
analysis
peptide purification by
HPLC
sequencing of
peptide
coupling to carrier protein for Ab
work
(usually crude peptide is fine) |
$ 50.00
$ 40.00
$ 68.00
$ 52.00
inquire
$ 100.00
$ 200.00 and up
$ 200.00
$ 100.00 |
We also offer site specific labeling of peptides with 2H, 3H, 13C, 14C
and 15N or biotin labeled amino acids--inquire about costs. D-amino
acids and other unusual amino acids can be incorporated into synthetic
peptides. Post synthesis modifications are availiable upon request.
The costs for all of these services are directly related to
the cost of these reagents.
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PROTEIN/PEPTIDE SEQUENCING:
N-TERMINAL
ANALYSIS
Our instruments perform automated Edman chemistry. The
PTC-amino acids generated are analyzed using a reverse-phase HPLC
system. Samples can be applied in solution to a polyamine treated glass
filter or pre-bond to certain nylon membranes. Up to forty residue can
be read but runs of less than 25 cycles are preferred. We like to
receive samples of greater than 20 pmoles.
Set-up
fee
per-cycle charge -3
cycle minimum |
$ 50.00
$ 20.00 |
If sample has the N-terminus blocked, hence cannot be sequenced by this
method the run is terminated after 3 cycles. $110.00 is charged for
these samples.
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PROTEOMICS:
As of January 2006 the K-State Core lab will have the ability to
analyze both purified proteins and isolated peptide fragments with our
new mass spectrometers. We have the ability to analyzed
proteins
by both standard 2d-PAGE and nanoflow HPLC. For more details
on
the system we purchased as well as its capabilities -- click on this link.
Due to the varied techniques available please inquire for pricing.
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MASS SPECTROMETRY
Click here to learn
more about about our Mass Spectrometry capabilities.
As of May 1995 we have been accepting samples for mass analysis of
molecules ranging from 500 -- `60,000 Da. The sensitivity of our laser
desorption, time of flight instrument is sub-picomole for masses under
20,000 Da and about 10 pmol for masses larger than 20,000 Da. Our mass
accuracy is within 1 AMU for molecules smaller than 5000 Da and
approximately 1% for higher masses. Samples should be relatively
salt-free. The cost per analysis is $ 20.00 for single samples. Our
facility will make every effort to accommodate your needs. Please feel
free to come by and discuss your particular project and/or problems.
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SURFACE
PLASMON RESONANCE:
Click here to learn more about
the Biacore 3000 instrument.
The Biacore 3000 instrument was recently acquired to give the core lab
a new dimension, the quantification of molecular interactions. This
instrument uses a treated gold slide that allows the covalent
attachment and display of lipids or ligands in a flow cell. Potential
binding partners can be introduced in solute and the subsequent binding
to the bound ligand can be measured. Using this technique on-rates and
off-rates can be measured in real time with minimal consumption of
materials. Using these values accurate association constants can be
determined. The charge for this instrument is $75.00 / day and the
researcher must purchase all of their materials and buffers.
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